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dck [2018-03-15 11:43:24]
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dck [2018-03-15 11:43:24]

deoxyadenosin/deoxycytidine kinase
locus
BSU00140
pI
5.54
mw
25.30 kDa
protein length
217 aa Sequence Blast
gene length
651 bp Sequence Blast
function
purine salvage and interconversion
product
deoxyadenosin/deoxycytidine kinase
essential
no
synonyms
yaaF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.3|Purine salvage and interconversion]

    • Gene

    Coordinates
    22,496 → 23,149

    RNA

    Catalyzed reaction/ biological activity

  • ATP + deoxyadenosine = ADP + dAMP. (accoding to SwissProt)
  • NTP + deoxycytidine = NDP + dCMP. (accoding to SwissProt)

    • The protein

    Structure

  • [PDB|2JAQ] (DAK from Mycoplasma mycoides, 35% identity) [pubmed|17229440]

    • Expression and Regulation

    Operons

    genes
    [gene|0DA340B3D23B63C6BD284BF86409558A31B12B53|dgk]-[gene|D749382783437F40AE1C66755F57D44E3FCA193F|dck]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B887 (yaaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1886 NBRP B. subtilis, Japan]
  • BKE00140 (Δ[gene|D749382783437F40AE1C66755F57D44E3FCA193F|dck]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCTTAGGGATATGATGTT, downstream forward: _UP4_TGAATCTCAGTCGAGATTCA
  • BKK00140 (Δ[gene|D749382783437F40AE1C66755F57D44E3FCA193F|dck]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCTTAGGGATATGATGTT, downstream forward: _UP4_TGAATCTCAGTCGAGATTCA

    • References

  • 6251049,11078735,17229440